A new stability-indicating RP-HPLC method for the determination of dicyclomine hydrochloride and dimethicone combination in tablet dosage forms

Abstract Background We describe a “stability-indicating liquid chromatography” technique for the estimation of dimethicone (DEC) and dicyclomine hydrochloride (DEH) in established tablet formulations. Individual quantification DEH DEC was reported. But simultaneous lacking. were analysed on an “XTerra C 18 column (250 mm × 4.6 mm, 5 μm)” with mobile phase solvent run isocratically 0.1M K 2 HPO 4 -acetonitrile (55:45, v/v) flow speed 1.0 mL/min. Results The chromatographic period assay 6.0 min retention times 2.134 2.865 min, respectively. method validated accuracy (99.453 to 100.417% 99.703 100.303% recovery values DEC, respectively), precision (RSV value 0.135% 0.171% DEH), linearity (5–15 μg/mL 20–60 DEC), selectivity (no hinderance from excipients) specificity degradants) recovery. Conclusion developed stability-indicating chromatography process well applied